Rajan Lamichhane’s Google Scholar Page
2026
Schuck, R., Ward, A., Barrera, F.N., Lamichhane, R. SiMPull-POP: Quantification of Membrane Protein Assembly via Single Molecule Photobleaching. Bio-protocol, 2026, 16(1): e5560. PubMed Link PDF
2025
Ward, A., Baeza-Ballesteros, L.B., Schuck, R.J., García-Murria, M.J., Lamichhane, R., Mingarro, I, Barrera, F.N. Cholesterol promotes the formation of dimers and oligomers of the receptor tyrosine kinase ROR1. J. Biol. Chem., 2025, Online, PubMed Link PDF
Gurumoorthy, V., Hicks, A., Tiruvadi-Krishnan, S., Leite, W.C., Chodankar, S., Koalpe, J.L., Smith, J.S., Lamichhane, R., O’Neill, H. The N-terminal domain of COMPANION OF CELLULOSE SYNTHASE1 promotes microtubule array formation in Arabidopsis . Plant. Physiol., 2025, 199, 1, 1-15 PubMed Link PDF
Mattamira, C., Ward, A., Tiruvadi-Krishnan, S., Lamichhane, R., Barrera, F.N., Sgouralis, I. Bayesian analysis and efficient algorithms for single-molecule fluorescence data and step counting. Biophys. J., 2025, 124, 1-13 PubMed Link PDF
Liu, T., Naidoo, N.R., Agyemang, E., Lamichhane, R. Insights into the structural dynamic of the secretin family (Class B1) G protein-coupled receptors. J. Biol. Chem., 2025, 301 (8) 110466 PubMed Link PDF
Agyemang, E., Lamichhane, R., A tale of detergent tails: GPCR activation beyond ligands. Structure., 2025, 33, 633-635 PubMed Link PDF
Lamichhane, R., Single-Molecule Insights into GPCR Conformational Landscapes. J Membr Biol., 2025, 258, 113-120 PubMed Link PDF
Schuck, R., Ward, A., Sahoo, A.R., Rybak, J., Pyron, R., Trybala, T., Simmons, T., Bacile, J., Sgouralis, I., Buck, M., Lamichhane, R.*, and Barrera, F.N*. Cholesterol inhibits assembly and oncogenic activation of the EphA2 receptor. Commun Biol., 2025, 8, 411 (* Co-corresponding authors) PubMed Link PDF
2024
Agyemang, E., Gonneville, A., Tiruvadi-Krishnan, S., and Lamichhane, R., Exploring GPCR conformational dynamics using single-molecule fluorescence. Methods., 2024, 226, 35-48 PubMed Link PDF
2023
Wei, S., Pour, N.G., Tiruvadi-Krishnan, S., Ray, A.P., Thakur, N., Eddy, M.T., and Lamichhane, R., Single-molecule visualization of human A2A adenosine receptor activation by a G protein and constitutively activating mutations. Commun Biol., 2023, 6, 1218 PubMed Link PDF
Liu, T., Khanal, S., Hertslet, G.D., Lamichhane, R., Single-molecule analysis reveals that a glucagon-bound extracellular domain of the glucagon receptor is dynamic. J Biol Chem., 2023, 299(9), 105160, PubMed Link PDF
2022
Thakur, N., Wei, S., Ray, A.P., Lamichhane, R.*, and Eddy, M.T.*, Production of human A2AAR in lipid nanodiscs for 19F-NMR and single-molecule fluorescence spectroscopy. STAR Protoc., 2022, 16;3(3):101535 (* Co-corresponding authors) PubMed Link PDF
2021
Wei, S., Thakur, N., Ray, A.P., Beining, J., Obeng, S., McCurdy, C.R., McMahan, L.R., Gutiérrez-de-Terán, Eddy, M.T., and Lamichhane, R. Slow conformational dynamics of the human A2A adenosine receptor are temporally ordered. Structure, 2021, 30, 329-337 PubMed Link PDF
Xi, J., Yang, N., Perez-Aguilar, J.M., Selling, B., Grothusen, J., Lamichhane, R., Saven, J.G., and Liu, R. Novel Variants of Engineered Water Soluble mu Opioid Receptors with Extensive Mutations and Removal of Cysteines. Proteins, 2021, 89, 1386-1393 PubMed Link PDF
Deng, Y.S., Hammond, J., Pauszek, R., Ozog, S., Chai, I. 2 , Rabuck-Gibbons, J., Lamichhane, R., Henderson, S.C., Millar, D.P., Torbett, B.E., and Williamson, J.R., Discrimination between functional and non-functional cellular Gag complexes involved in HIV-1 assembly. J Mol Biol, 2021, 433 (8), 166842 (1-21) PubMed Link PDF
Pauszek, R.F., Lamichhane, R., Rajkarnikar Singh, A., and Millar, D.P. Single-molecule view of coordinationin a multi-functional DNA polymerase. eLife, 2021, 10: e62046, PubMed Link PDF
Stefanski, K.M., Russell, C.M., Westerfield, J.M., Lamichhane, R.*, and Barrera, F.N., PIP2 promotes conformation-specific dimerization of the EphA2 membrane region. J Biol Chem, 2021 296 (100149):1-12 (* Co-corresponding authors) PubMed Link PDF
2020
Lamichhane, R., Liu J.J., White, K.L., Katritch, V., Wüthrich, K., Stevens, R.C., and Millar, D.P. Biased Signaling of the G-Protein-Coupled Receptor β2AR Is Governed by Conformational Exchange Kinetics. Structure, 2020, 28, 1-7. PubMed Link PDF
2019
Lamichhane, R., How Proteins Recognize RNA. Biophysics of RNA-Protein Interactions. Biological and Medical Physics, Biomedical Engineering, 2019, 3-21. SpringerLink PDF
2017
Hammond, J.A., Zhou, L., Lamichhane, R., Chu, HY., Millar, D.P., Gerace, L., and Williamson, J.R. A Survey of DDX21 activity during Rev/RRE complex formation. J Mol Biol, 2017, 430, 537-553. PubMed Link PDF
Lamichhane, R., Liu J.J., Pauszek, R.F III, and Millar, D.P. Monitoring conformational changes in a G protein-coupled receptor at the single-molecule level using a Cy3 fluorescence probe. Bio-protocol, 2017, 7, 12, e2332. PubMed Link PDF
Lamichhane, R., Hammond, J.A., Pauszek, R.F. III, Anderson, R., Pedron, I., van der Schans, E., Williamson, J.R., and Millar, D.P. The DEAD-Box protein DDX1 acts through RNA to promote HIV-1 Rev-RRE assembly. Nucleic Acids Res, 2017, 45, 4632-4641.PubMed Link PDF
Hammond, J.A., Lamichhane, R., Millar, D.P., and Williamson, J.R., A DEAD-box helicase mediates an RNA structural transition in the HIV-1 Rev response element. J Mol Biol, 2017, 429, 697-714. PubMed Link PDF
Lamichhane, R., Mukherjee, S., Smolin, N., Pauszek, R., Bradley, M., Sastri, J., Robia, S.L., Millar, D.P., and Campbell, E.M. Conformational changes in the rhesus TRIM5α dimer dictate the potency of HIV-1 restriction. Virology, 2017, 500, 161-68. PubMed Link PDF
2016
Baker, K.A.*, Lamichhane, R.*, Rueda, D., and Cunningham, P.R. Protein-RNA dynamics in the central junction control 30S ribosome assembly. J Mol Biol, 2016, 428, 3615-31. (* Equal Authorship) PubMed Link PDF
Lavergne, T.*, Lamichhane, R.*, Malyshev, D., Li, Z., Li, L., Sperling, E., Williamson, J., Millar, D., and Romesberg, F. FRET characterization of complex conformational changes in a large 16S ribosomal RNA fragment site-specifically labeled using unnatural base pairs.ACS Chem Biol, 2016, 11, 1347-53.(* Equal Authorship) PubMed Link PDF
2015
Lamichhane, R.* Liu J.J.*, Pljevaljcic, G., van der Schans, E., Katritch, V., Wüthrich, K., Stevens, R.C., and Millar, D.P. Single-molecule view of basal activity and activation mechanisms of the G protein-coupled receptor b2AR. PNAS, 2015, 112, 14254-59. (* Equal Authorship) (featured in TSRI News and Views) PubMed Link PDF
2013
Lamichhane, R., Berezhna, S.Y., Gill, P.J., Van der Schans, E., and Millar,D.P. Dynamics of site switching in DNA polymerase I. JACS, 2013, 135, 4735-42. PubMed Link PDF
2012
Berezhna, S.Y., Gill, J., Lamichhane, R., and Millar, D.P. Single-molecule FRET reveals an innate fidelity checkpoint in DNA polymerase I. JACS, 2012, 134, 11261-68. PubMed Link PDF
Salim, N.*, Lamichhane, R.*, Zhao, R., Banarjee, T., Philip, J., Rueda, D., and Feig, A.L. Thermodynamic and kinetic analysis of an RNA kissing interaction and its resolution into an extended Duplex. Biophysical Journal, 2012, 102, 1097-1106. (* Equal Authorship) PubMed Link PDF
2010
Zhao, R., Marshall, M., Aleman, E., Lamichhane, R., Feig, A., and Rueda, D. Laser-induced single-molecule temperature jump. Biophysical Journal,2010, 99, 1925-31. PubMed Link PDF
Lamichhane, R., Solem, A., Black, W., and Rueda, D. Single molecule FRET of nucleic acid-protein and protein-protein interactions: surface passivation and immobilization. Methods, 2010, 52, 192-200. PubMed Link PDF
Lamichhane, R., Daubner, G., Thomas-Crusells, J., Auweter, S.D., Manatchal, C., Austin, K.S., Vaniuk, O., Allain, F.H., and Rueda, D. RNA looping by PTB: evidence using FRET and NMR spectroscopy and for a role in splicing repression. PNAS, 2010, 107, 4105-10 (featured by faculty of 1000). PubMed Link PDF
2008
Aleman, E. A., Lamichhane, R., and Rueda, D. Exploring RNA folding one molecule at a time. Curr Opin Chem Biol, 2008, 12, 647-54. PubMed Link PDF
2005
Thuy, M.L., Kharel, M.K., Lamichhane, R., Lee, H.C., Suh, J.W., Liou, K.K., and Sohng, J.K. Expression of 2-deoxy-scyllo-inosose synthase (kanA) from kanamycin gene cluster in Streptomyces lividans. Biotechnology Letters 2005, 27, 465-70. PubMed Link PDF
2004
Sthapit, B., Oh, T.J., Lamichhane, R., Liou, K.K., Lee, H.C., Kim, C.G., and Sohng, J.K. Neocarzinostatin naphthoate synthase: an unique iterative type I PKS from neocarzinostatin producer Streptomyces carzinostaticus. FEBS Letters 2004,566, 201-6. PubMed Link PDF
The Lamichhane Lab 